Cooperative Treatment of Gastric Cancer Using B7-H7 siRNA and Docetaxel; How Could They Modify Their Effectiveness?

Purpose: Despite the high prevalence of gastric cancer (GC), drug resistance is a major problem for effective chemotherapy. B7-H7 novel member B7 superfamily and expressed in most common cancers. However, role on aggressiveness GC chemosensitivity has remained unknown. Therefore, this study was designed to assess effect suppression using small interference RNA (siRNA) combination with docetaxel cells. Methods: MTT test applied determine IC50 combined siRNA viability MKN-45 To B7-H7, BCL-2, BAX, caspase-3-8-9 genes expression, qRT-PCR performed. Furthermore, flow cytometry evaluate apoptosis cell cycle status. Finally, therapy migratory capacity colony-forming ability, wound healing assay colony formation were employed, respectively. Results: increased chemo-sensitivity cells docetaxel. The expression mRNA reduced after Also, alongside migration rate, arrested at G2-M phase, induced by modulating apoptotic target genes. Conclusion: plays significant pathogenesis GC. suppression, docetaxel, may be promising therapeutic approach treating